Total phenolic content and biological activities of enzymatic extracts from Sargassum muticum (Yendo) Fensholt

Maya Puspita (1) , Maureen Déniel (1, 2) , Ita Widowati , Ocky Karna Radjasa , Philippe Douzenel (3) , Christel Marty (1) , Laurent Vandanjon (1) , Gilles Bedoux (1) , Nathalie Bourgougnon (1)

1 LBCM - Laboratoire de Biotechnologie et Chimie Marines
2 GEPEA - Laboratoire de génie des procédés - environnement - agroalimentaire
3 UBS Vannes - Université de Bretagne Sud - Vannes

Seaweeds are potentially excellent sources of bio- active metabolites that could represent useful leads in the de- velopment of new functional ingredients in pharmaceutical and cosmetic industries. In the last decade, new marine bioprocess technologies have allowed the isolation of sub- stances with biological properties. The brown alga Sargassum muticum (Yendo) Fensholt (Ochrophyta) was en- zymatically hydrolyzed to prepare water-soluble extracts by using six different commercially available carbohydrate- degrading enzymes and two proteases. Evaluation of 2,2- diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antiox- idant power (FRAP) antioxidant, tyrosinase, elastase, and bio- film inhibition, antibacterial and antiviral activities as well as evaluation of cytotoxicity were realized for each extract. Total phenolic content was measured for extract characterization, and solid-phase extraction was useful to purify the enzymatic extract. Soluble total phenolic content of S. muticum Viscozyme extract was highest with 6.4% of dry weight. Enzymatic Celluclast and Viscozyme extracts had the lowest value of DPPH IC50 indicating a strong antiradical activity, 0.6 mg mL−1, in comparison with other enzymes. The ferric reducing antioxidant power ranged between 48.7 μM Fe 2+ Eq, digested with Viscozyme, and 60.8 μM Fe 2+ Eq, digested with Amyloglucosidase. Tyrosinase inhibition activity of S. muticum Neutrase extract was 41.3% higher compared to other enzymes. Elastase inhibition activity of S. muticum Shearzyme extract had highest activity (32.8%). All enzymat- ic extracts showed no cytotoxic effect towards the kidney Vero cells. Meanwhile, only S. muticum Neutrase and Alcalase ex- tracts exhibited potential antiviral activity. In addition, S. muticum Viscozyme and Shearzyme extracts showed prom- ising activity in suppressing the biofilm formation against Pseudomonas aeruginosa and Escherichia coli, respectively. Purification of S. muticum Viscozyme extracts by solid-phase extraction managed to concentrate the phenolic content and improve the bioactivity. These results indicate the promising potential of enzyme-assisted followed by solid-phase extrac- tion in recovering phenolic content and in improving its bioactivity.

Mots clés: Bioactivities; Phaeophyceae; Carbohydrase; Enzyme-assisted extraction; Protease; Solid-phase extraction

Domaines: Sciences du Vivant [q-bio]; Biologie végétale

Maya Puspita, Maureen Déniel, Ita Widowati, Ocky Karna Radjasa, Philippe Douzenel, et al.. Total phenolic content and biological activities of enzymatic extracts from Sargassum muticum (Yendo) Fensholt. Journal of Applied Phycology, 2017, 29 (5), pp.2521-2537. ⟨10.1007/s10811-017-1086-6⟩. ⟨hal-02115032⟩